NRF2 Inhibits Cardiomyocyte Pyroptosis Via Regulating CTRP1 in Sepsis-Induced Myocardial Injury.

ABSTRACT: C1q/tumor necrosis factor-related protein 1 (CTRP1) has been demonstrated as a crucial regulator in myocardial injury (MI). The present study aims to evaluate the mechanism of CTRP1 in sepsis-induced MI. The septic mouse model was established via cecal ligation and puncture and the in vitro cell model was established via lipopolysaccharide treatment. The mouse survival rate within 96 h was recorded. Morphologic changes of cardiomyocytes were observed and cell viability and cardiac functions were detected. CTRP1 and nuclear factor erythroid 2-related factor (Nrf2) expressions, creatine troponin-T, and creatine phosphokinase isoenzyme levels, and expressions of pyroptotic markers were determined. The binding relationship between Nrf2 and the CTRP1 promotor was predicted and verified. Rescue experiments were designed to confirm the role of CTRP1. CTRP1 was poorly expressed in septic mice. CTRP1 overexpression inhibited cardiomyocyte pyroptosis and improved cardiac functions, MI, and survival rate in septic mice. Nrf2was decreased in cecal ligation and puncture -treated mice. Nrf2 overexpression promoted CTRP1 expression via binding to the CTRP1 promotor and suppressed cardiomyocyte pyroptosis. CTRP1 downregulation abolished the inhibitory effect of Nrf2 overexpression on cardiomyocyte pyroptosis. Overall, Nrf2 promoted CTRP1 expression via binding to the CTRP1 promotor to inhibit cardiomyocyte pyroptosis, thereby alleviating MI in septic mice.

Prevalence and risk factors of hand eczema in hospital-based nurses in northern China.

BACKGROUND/OBJECTIVES: This study aimed to evaluate the prevalence of hand eczema among nurses in northern China and to analyse associated risk factors. METHODS: A retrospective self-reported questionnaire was designed and distributed to 1322 nurses who were selected from three regional hospitals. Risk factors related to hand eczema in nurses were analysed using multivariable logistic regression. RESULTS: Hand eczema was present in 183/934 respondents (20%) and was not significantly associated with gender or workplace (P > 0.05). Factors such as the use of moisturiser (odds ratio [OR] 0.6; 95% confidence interval [CI] 0.3-1.0) and not wearing impervious gloves (OR 0.5; 95% CI 0.3-0.7) were associated with lower odds of developing hand eczema. A personal (OR 5.7; 95% CI 3.9-8.2) and family history (OR 2.3; 95% CI 1.5-3.5) of atopy, washing hands more than 20 times per day (OR 1.6; 95% CI 1.1-2.4), and more years working as a nurse (OR 1.1; 95% CI 1.0-1.1) were associated with higher odds of developing hand eczema. CONCLUSIONS: A personal and familial history of atopy, the prolonged use of impervious gloves, washing hands more than 20 times per day and more years in work as a nurse are all risk factors for increased hand eczema in nurses.

[In-situ FTIR study of CO adsorption on Co-Mo/Al2O3 hydrodesulphurization catalysts].

Due to the implementation of more stringent specifications in sulfur content for diesel oil, a deep understanding of the active phase of Co-Mo/Al2O3 catalysts is necessary to the development of ultra-deep hydrodesulphurization (HYD) catalysts. A series of reductive Co-Mo/Al2O3 catalysts prepared in the lab and the high-active industrial catalyst (G) were studied by in-situ FTIR using CO as probe molecule. The showed a good relationship with the desulphurization activities of the catalysts. With the increase in MoO3 and CoO loading, the desulphurization activity of catalyst increases, and the infrared spectrum changes with the amount of CO adsorbed on the catalyst. There is a new band at 2179 cm(-1) when the MoO3 loading is up to 20% and CoO up to 4. 16%. According to the activities of the catalysts, the appearance of this new band suggests that the catalyst has higher hydrodesulphurization (HYD) activity. Compared with the infrared spectrum of CO on the catalyst of the same MoO3 and CoO loading by the common preparation method, the catalyst prepared with chelating agents has higher HYD activity and its band at 2 179 cm(-1) is stronger. The infrared spectrum of CO adsorbed on optimum Co-Mo/Al2O3 catalyst prepared in the lab is similar to the highly active industrial catalyst (G). Their bands at 2179 cm(-1) are both very strong and their HYD activities are both higher than the others. Thus, the appearance and the increase of the band at 2179 cm(-1) indicate the increase in the HYD activity of Co-Mo/Al2O3 catalysts to some extent, which could be an effective tool for developing ultra-deep HYD catalysts.